A simple, precise, accurate, reproducible and robust HPLC method was developed for the estimation of curcumin whole extract. The method was validated as per ICH guidelines. Analysis of the drug was performed on Phenomenex C18 (250 mm x 4.6 mm, 5 μ) column using 0.1 % citric acid: acetonitrile in a gradient elution system (0.01 – 8.00 min = 2.0 ml/min, 8.00 – 18.00 min = 0.6 ml/min, 18.00 – 45.00 min = 2.0 ml/min). Curcuminoids were detected at 420 nm and turmerone was detected at 257 nm. Retention times for the components were observed as Bis-Demethoxy curcumin: 13.29 min, Desmethoxy curcumin: 14.19 min, Curcumin: 14.99 min and Ar-Turmerone: 24.67 min. Linearity was obtained in the range of 10-50 μg/ml. The % recovery was observed to be 99.72 – 100.30% for Bis-D-curcumin, 100.42 – 101.20% for D-curcumin, 98.85 – 100.57% for Curcumin and 99.04 – 100.19% for Ar-turmerone respectively. The statistical parameters were found within range.
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